Study on the Chemical Constituents and Biological Activity of Cosmos Sulphureus Leaf Extract

Alagbe John Olujimi

doi:10.59890/ijir.v3i10.93

Authors

Alagbe John Olujimi Department of Biochemistry, Gandhi College of Agriculture, Rajasthan, India

DOI:

https://doi.org/10.59890/ijir.v3i10.93

Keywords:

Cosmos Sulphureus, Leaf Extract, Phytochemicals, Chemical, Free Radicals, Stress

Abstract

This study was carried out to evaluate the chemical constituents and biological activity of Cosmos sulphureus leaf extract. Proximate analysis of Cosmos sulphureus leaf powder shows that it had a moisture content of 13.88 %, crude protein (6.91 %), crude fibre (11.92 %), ether extract (0.41 %), ash (12.09 %) and energy (1392.4 Kcal/kg). Qualitative analysis of Cosmos sulphureus leaf extract revealed the presence of phenolic compound, alkaloids, saponins, tannins, flavonoids, steroids and anthraquinones. GC-MS analysis reveals the presence of 35 bioactive compounds representing 89.18% (Triterpenes (50.07%), sesquiterpenes (23.96%), monoterpenes (7.33%), diterpenes (4.95%) and non-terpenes (2.87%)]. The predominant bioactive compounds in Cosmos sulphureus leaf extract were; 17-Octadecynoic acid (9.94%), γ-Elemene (9.65%), β-Selinenol (9.11%), 8-Heptadecene (8.52%), β-Caryophyllene oxide (8.74 %), Calarene epoxide (7.52%), 9-Hexadecenoicacid (6.52%), Benzene,1,2,3-trimethyl(5.46%), Squalene (5.02%). The extract delivers a broad-spectrum antimicrobial effect against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Shigella flexenari, Staphylococcus aureus, Candida albicans, Aspergillus niger and Penicillium notatum. Cosmos sulphureus leaf extract was also able to protect against oxidative stress and neutralize free radicals at the source. It was concluded that Cosmos sulphureus leaf extract have bioactive compounds that go far beyond nutrition and its medicinal powerhouse provides powerful: anti-inflammatory, antioxidant, hepato-protective, immuno-modulatory, gastro-protective, anti-helminthic, antidiarrheal, anti-tumor, antimicrobial, antiviral and antifungal properties

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